qPCR (real-time PCR) protocol explained

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John
Cinese (semplificato)
Studenti Universitari
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Riepilogo
qPCR（实时PCR）是一种有效的技术，用于定量确定样本中目标序列或基因的存在。它是检测病毒感染（如COVID-19）的金标准方法。通过提取RNA并逆转录为DNA，使用qPCR可以在光循环仪中分析DNA。与传统PCR不同，qPCR实时监测每个循环的产物积累，利用荧光探针生成经典的qPCR曲线。qPCR主要有两种方法：Cyber Green法和TaqMan法，分别通过不同的荧光信号实现DNA的定量检测。该技术广泛应用于诊断和基因表达分析。
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